Job Description

Responsibilities AAV Strategic responsibilities Produce research-grade AAV vectors using the HEK293 system and density gradient-based purification, use of cell stacks, cell factories, small bioreactors Enable execution of discovery and pre-clinical activities through the production of research grade AAV vectors. Support development of robust, well-characterized, high quality and high yielding downstream production processes for AAV gene therapy products across internal programs. Contribute to the design and execution studies to develop a thorough understanding of the process design space, with a view towards scale-up and successful regulatory filings. AAV Tactical responsibilities Clone highly complex library assemblies for AAV production Execute large scale DNA preparation protocols for transfection Implement next-gen sequencing DNA preparation procedures Set up and manage helper plasmids and AAV vectors inventories Co-ordinate production needs with different teams and manage supply chain Proactively communicate risks to delivery to management Help establish and implement protocols, SOPs and systems within the developing team Maintain electronic lab notebooks and communicate results to stakeholders Maintain requests dashboard and database Maintain production cell lines and their inventory Oversee and mentor laboratory technicians and interns in proper laboratory techniques and scientific experimental design Manufacturability group standard responsibilities Interact with peers across the organization including in the US Communicate key finding to internal and external stakeholders Assess the manufacturability of a client's modality in conjunction with the rest of the manufacturability team Engage collaboratively with the client to understand and improve the manufacturability of therapeutic modalities Author and follow standard operating procedures, batch records, and other process documentation Filing of invention disclosures and basic understanding of intellectual property with the assistance of relevant internal and external parties/stakeholders Drive innovation through the design and troubleshooting of experiments for testing various unit operations in process development in accordance with clients need and company's overall strategic goalsRequirements PhD in molecular biology, virology, synthetic biology or equivalent with 1-3 years of laboratory research experience 1-5 years of work experience with various AAV systems Good understanding of AAV analytics (ddPCR, ELISA, CE-SDS, AUC) in support of product characterization Innovative and analytical thinker with outstanding interpersonal, communication, and leadership skill Demonstrated ability to clearly communicate technical information and roadmaps through written protocols and/or presentations containing clean figures and visualizations Ability to work in BSL2 lab Work authorization in SingaporeDesired Qualifications Strong demonstrable experimental planning/troubleshooting skills Hands-on lab knowledge and experience with current and next-gen genome engineering and sequencing technologies Strong tissue culture skills
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