The National Institute of Education invites suitable applications for the position of Research Assistant on a 12-month contract at the
Project Title: Molecular biology of temperature-induced bleaching and subsequent recovery in the giant clam, Tridacna squamosa [RI6/22 CSF and RS5/23 CSF]
Project Introduction:
Giant clams are marine bivalve mollusks that live in symbiosis with extracellular symbiotic dinoflagellates (zooxanthellae), which are phototrophic. They inhabit shallow reef waters of the tropical Indo-Pacific. Tridacna squamosa, a species of giant clams can be found in Singapore waters, albeit with dwindling populations. Due to the rise in ocean temperature, giant clams like scleractinian corals can undergo bleaching i.e., they lose their symbionts and become whitish in color. However, studies have revealed that eight months after the bleaching event, the mortality rate of the bleached clams under natural conditions was
5%, while those of some coral species under the same conditions were
99%. Thus, giant clams have a higher tolerance to bleaching and can recover from bleaching better than corals. Currently, there is a dearth of knowledge on the molecular biology of bleaching and the subsequent recovery process in giant clams. Therefore, the objective of this study is to use biochemical, molecular (including cloning, sequencing, quantitative real-time polymerase chain reaction, western blotting) and immunohistochemical methods to elucidate the mechanisms and processes related to bleaching and recovery from bleaching in T. squamosa.
Requirements
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