Job Description

Research Officer position
A research officer position is available immediately in the laboratory of Dr. Leslie Beh at IMCB.
The Beh lab seeks to develop cutting-edge CRISPR-Cas technologies to uncover the
biological functions of RNA modifications. Dysregulation of these modifications has been
linked to cancer, neurological disorders, and metabolic disease. We will determine the
mechanisms by which RNA modifications operate by drawing upon diverse techniques,
including protein engineering, biochemical reconstitution, genomics, and cell-based
experiments. Successful applicants can expect close mentorship in a collaborative
environment, with an emphasis on developing scientific knowledge and individual career goals.
Requirements:

• B.Sc. (Hons) or Diploma from a well-recognized institution in Life Sciences / Biomedical

Sciences

• Experience in molecular biology techniques, including cloning, DNA and RNA purification,

and protein expression / purification

• Enthusiastic and skilled at solving difficult, multi-disciplinary problems
• Excellent organizational and communication skills

Preferred experience:

• Mammalian cell culture, especially with mouse and human embryonic stem cells
• NGS library generation and data analysis
• Prior roles in lab management

Interested candidates are asked to send a cover letter detailing your research motivations, CV, and the names and email addresses of three references to Dr. Leslie Beh (lesliebeh@gmail.com).
Publications:
Hoffmann, F.*, Kim, M.*, Beh, L.Y.*, Wang, J., Vo, P.L.H., Gelsinger, D.R., Acree, C., Mohabir,
J.T., Fernandez, I.S., Sternberg, S.H. Selective recruitment of the AAA+ ATPase TnsC
increases the fidelity of Type I-F CRISPR RNA-guided transposition. Nature (in press).

• Co-first author

Beh, L.Y., Debelouchina, G.T., Clay, D.M., Thompson, R.E., Lindblad, K.A., Hutton, E.R.,
Bracht, J.R., Sebra, R.P., Muir, T.W., Landweber, L.F. Identification of a DNA methyltransferase
complex and the impact of N6-methyladenine on chromatin organization.
Cell. 177(7): 1781-96. Cover story
Beh, L.Y., Muller, M.M., Muir, T.W., Kaplan, N., Landweber, L.F. DNA-guided establishment
of nucleosome patterns within coding regions of a eukaryotic genome. Genome Res.
25(11): 1727-38.
Beh, L.Y., Colwell, L.J., Francis, N.J. A core subunit of Polycomb repressive complex 1 is
broadly conserved in function but not primary sequence. Proc Natl Acad Sci U S A. 109:
E1063-71.
Klompe, S.E., Jaber, N., Beh, L.Y., Mohabir, J.T., Sternberg, S.H. Evolutionary and
mechanistic diversity of Type I-F CRISPR-associated transposons. Mol Cell. 82(3): 616-628.
Feng, Y., Beh, L.Y. SIGAR: Inferring Features of Genome Architecture and DNA
Rearrangements by Split-Read Mapping. Genome Biol Evol. 12(10): 1711-18.
Wojcik, F., Dann, G.P., Beh, L.Y., Debelouchina, G.T., Hofmann, R., Muir, T.W. Functional
crosstalk between histone H2B ubiquitylation and H2A modifications and variants. Nat
Commun. 9(1): 1394.
Chen, X., Jung, S., Beh, L.Y., Eddy, S.R., Landweber, L.F. Combinatorial DNA rearrangement
facilitates the origin of new genes in ciliates. Genome Biol Evol. 7(10): 2859-70.
The above eligibility criteria are not exhaustive. A*STAR may include additional selection criteria based on its prevailing recruitment policies. These policies may be amended from time to time without notice. We regret that only shortlisted candidates will be notified.